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KMID : 0603520030080030166
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Journal of Korean Association of Cancer Prevention
2003 Volume.8 No. 3 p.166 ~ p.172
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Overexpression of Adenine Nucleotide Translocase Induces Apoptosis in Drosophila Cultured Cell
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Shin Myoung-Joo
Park So-Young
Yoo Mi-Ae
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Abstract
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The adenine nucleotide translocase (ANT) is a major component of mitochondrial permeability transition pore (MPTP) and catalyzes the exchange of adenine nucleotides across the mitochondrial inner membrane. It has recently been known that ANT is required for apoptosis mediated by Bax in mammal. However, in Drosophila, the study of ANT function is rarely known. The Drosophila ANT genes are duplicated tandemly, sesB and Ant2 respectively. In this study, using a GAL4-UAS system, we investigated the gain-of-function of sesB and Ant2 gene in Drosophila Kc cells. We confirmed overexpression of sesB and Ant2 gene in Drosophila Kc cell transiently transfected with the constructed plasmids pUAST-sesB or pUAST-Ant2, and pAc-GAL4 through RT-PCR. Subsequently, we observed apoptosis in Drosophila cultured cells transfected with the plasmid pAc-GAL4/pUAST-sesB or pAc-GAL4/pUAST-Ant2. The present results indicate that Drosophila ANT overexpression lead to apoptosis and suggest that dysregulation of ANT expression may be also associated with apoptosis.
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KEYWORD
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Adenine nucleotide translocase(ANT), Apoptosis, GAL4-UAS system, Drosophila cultured cell, Adenine nuclcotide translocase(ANT)
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